Prasad Thazhe Kootteri

Selected Projects & Achievements

• Designed antiviral compounds using computational and crystallographic methods, achieving sub-micromolar Kd/IC₅₀
• Developed a dual-target inhibitor by integrating structural data to enhance selectivity and potency
• Targeted BRCA1 tBRCT domain via NMR-based fragment screening and structure-guided expansion
• Led fragment screening across diverse protein families, enabling rational probe development
• Identified binding pockets via protein backbone NMR assignment
• Characterized small molecule–protein interactions using mass spectrometry
• Integrated structural biology with biophysical validation to accelerate probe design

Small Heat Shock Proteins (sHsps)

• Investigated the structure and function of sHSPs and HSP90 using molecular biology, biophysics, and biochemistry
• Employed SEC-MALS, DLS, analytical SEC, and CD to study protein oligomerization

Materials Chemistry Projects

Synthesis and Application of Porous Metal-Organic Frameworks (MOFs)

• Utilized techniques in inorganic, organic, and supramolecular chemistry, along with X-ray crystallography, to characterize organic ligands and coordination complexes
• Characterized porous MOFs, determined crystal structures, and examined gas sensing and adsorption properties post-synthetic modification
• Designed, synthesized, and characterized ligands via multi-step organic synthesis
• Maintained and troubleshot laboratory instruments including single crystal X-ray diffractometers, gas adsorbers, and elemental analyzers

Structures of Polyoxometalates

• Collected single crystal X-ray data and performed structure solution and refinement of giant polyoxometalate clusters

Synthesis, Structure, and Application of Single Molecule Magnets (SMMs)

• Characterized SMMs and determined crystal structures
• Designed, synthesized, and purified organic ligands through multi-step synthesis

Synthesis of Metal-Based Drugs

• Synthesized and structurally characterized bismuth and lanthanide-based coordination compounds
• Trained and guided a junior research student throughout the project

Post-Doc (Protein crystallography, Biophysics, Molecular biology)
Indian Institute of Science, Molecular Biophysics Unit, Bangalore, India
2014 - 2017

Post-Doc (Inorganic, Organic synthesis, Supramolecular chemistry)
Seoul National University, Department of Chemistry Seoul, Republic of Korea
2013 - 2014 | 2011 - 2012 | 2008 - 2009

Post-Doc (Inorganic chemistry, Crystallography)
Bielefeld University, Department of Chemistry Bielefeld, Germany
2012 - 2013

Post-Doc (Inorganic, Organic Synthesis, Molecular Magnetism)
UNIMORE , Department of Chemical and Geological Sciences Moderna, Italy
2010 - 2011

Post-Doc (Main group chemistry, Metal-based drugs, Crystallography)
University of Hyderabad , School of Chemistry Hyderabad, India
2008

• S. Jos, A. Kambaru, T. K. Prasad, S. Parthasarathi, N. Kamariah, S. Nath, B. Padmanabhan, S. Padavattan, Structural and functional insights into the nuclear role of Parkinson’s disease-associated α-synuclein as a histone chaperone. Communications Biology 2025, 8, 712.
• V. Kumar, P. K. Chunchagatta Lakshman, T. K. Prasad, K. Manjunath, S. Bairy, A. S. Vasu, B. Ganavi, S. Jasti, N. Kamariah, Target-based drug discovery: Applications of fluorescence techniques in high throughput and fragment-based screening. Heliyon 2024, 10, e23864.
• R. Kavya, A. Snehal, S. Jos, T. K. Prasad, K. N. Kumuda, S. Unni, B. Padmanabhan, N. Kamariah, S. Padaanabhan, R. B. Mythri, High-affinity binding of celastrol to monomeric α-synuclein mitigates in vitro aggregation. J. Biomol. Struct. Dyn. 2023, 41, 12703-12713.
• S. Jos, H. Gogoi, T. K. Prasad, M. A. Hurakadli, N. Kamariah, B. Padmanabhan, S. Padavattan, Molecular insights into α-synuclein interaction with individual human core histones, linker histone, and dsDNA. Protein. Sci. 2021, 30, 2121-2131.
• A. E. Bryn, S. Hardwick, A. T. Crooks, N. Milech, P. M. Watt, C. Mithra, V. Kumar, S. Giridharan, G. Sadasivam, S. Mathivanan, S. Sudhakar, S. Bairy, K. Bharatham M. A. Hurakadli, T. K. Prasad, N. Kamariah, M. Muellner, M. Coelho, C. J. Torrance, G. J. McKenzie, A. R. Venkitaraman, Target identification for small-molecule discovery in the FOXO3a tumor-suppressor pathway using a biodiverse peptide library. Cell Chem. Biol. 2021, 28, 16052-1615.
• T. K. Prasad, M. P. Suh, Synthesis of multifunctional metal–organic frameworks and tuning the functionalities with pendant ligands. Dalton Trans. 2020, 49, 15034-15040.
• T. K. Prasad, K. Suguna, Sequence and Temperature Dependent Oligomers of the Small Heat Shock Protein HSPB1 from Zebrafish (Danio rerio). 2018.
• T. K. Prasad, M. P. Suh, Metal-Organic Frameworks Incorporating Various Alkoxy Pendant Groups: Hollow Tubular Morphologies, X-ray Single-Crystal Structures, and Selective Carbon Dioxide Adsorption Properties. Chem. As. J. 2015, 10, 2257-2263.
• A. Muller, S. Garai, C. Schaffer, A. Merca, H. Bogge, A. J. M. Al-Karawi, T. K. Prasad, Water Segregation/Repellency in Hydrophobic Nanocapsules. Chem. Eur. J. 2014, 20, 6659-6664.
• T. K. Prasad, M. V. Rajasekharan, Novel three-dimensional coordination polymers of lanthanides with sulfate and oxydiacetic acid. Acta Cryst. 2013, C69, 1503-1508.
• T. K. Prasad, M. P. Suh, Control of Interpenetration and Gas-Sorption Properties of Metal-Organic Frameworks by a Simple Change in Ligand Design. Chem. Eur. J. 2012, 18, 8673-8680.
• T. K. Prasad, G. Poneti, L. Sorace, M. J. Rodriguez-Douton, A.-L. Barra, P. Neugebauer, L. Costantino, R. Sessoli, A. Cornia, Magnetic and optical bistability in tetrairon(III) Single Molecule Magnets functionalized with azobenzene groups. Dalton Trans. 2012, 41, 8368-8378.
• M. P. Suh, H. J. Park, T. K. Prasad, D.-W. Lim, Hydrogen Storage in Metal-Organic Frameworks. Chem. Rev. 2012, 112, 782-835.
• O. O. E. Onawumi, O. O. P. Faboya, O. A. Odunola, T. K. Prasad, M. V. Rajasekharan, Mixed ligand tris-chelate of copper(II) with N,N-donor ligands - Synthesis, structure and spectra of [Cu(en)(phen)2]X2 2phen 8H2O (X = Cl-, NO3-). Polyhedron 2011, 30, 725-729.
• T. K. Prasad, D.-H. Hong, M. P. Suh, High Gas Sorption and Metal-Ion Exchange of Microporous Metal-Organic Frameworks with Incorporated Imide Groups. Chem. Eur. J. 2010, 16, 14043-14050.
• O. Anjaneyulu, T. K. Prasad, K. C. Kumara Swamy, Tris(4-oxy-pyridinium)nitrato lanthanide complexes [M(4-O-C6H4NH)3(NO3)2(H2O)2] [NO3] {M = La, Ce, Pr, Nd, Eu, Gd}- Synthesis, properties and structural characterization. Inorg. Chim. Acta. 2010, 363, 2990-2995.
• T. K. Prasad, M. V. Rajasekharan, Heterometallic coordination compounds of dipicolinic acid with cerium(III, IV) and copper(II). Synthesis, crystal structure and spectral studies. Inorg. Chim. Acta. 2010, 363, 2971-2976.
• O. Anjaneyulu, T. K. Prasad, K. C. Kumara Swamy, Coordinatively polymeric and monomeric bismuth(III) complexes with pyridine carboxylic acids. Dalton Trans. 2010, 39, 1935-1940.
• T. K. Prasad, M. V. Rajasekharan, Cerium(IV)-Lanthanide(III)-Pyridine-2,6-dicarboxylic Acid System: Coordination Salts, Chains, and Rings. Inorg. Chem. 2009, 48, 11543-11550.
• P. K. Poddutoori, P. Poddutoori, B. G. Maiya, T. K. Prasad, Y. Kandrashkin, S. Vassiliev, D. Bruce, A. v. d. Est, Redox control of photo-induced electron transfer in axial terpyridoxy porphyrin complexes. Inorg. Chem. 2008, 47, 7512-7522.
• T. K. Prasad, M. V. Rajasekharan, Solvent Dependent Crystallization of Isomeric Chain Coordination Polymers in the Ce-Zn/Cd-dipic System. Cryst. Growth. Des. 2008, 8, 1346-1352.
• T. K. Prasad, M. V. Rajasekharan, Synthesis and Structural Characterisation of a Mixed-valent Cerium Complex Containing a (H2O)38 Cluster. Synthesis and Reactivity in Inorganic, Metal-Organic, and Nano-Metal Chemistry 2008, 38, 55-61.
• O. O. E. Onawumi, O. O. P. Faboya, O. A. Odunola, T. K. Prasad, M. V. Rajasekharan,Synthesis, structure and spectral studies on mixed ligand copper(II) complexes of diimines and acetylacetonate. Polyhedron 2008, 27, 113-117.
• T. K. Prasad, J.-P. Costes, M. V. Rajasekharan, A Cubic 3d-4f Structure with Only Ferromagnetic Gd-Mn Interactions. Angew. Chem. Int. Ed. 2007, 46, 2851-2854.
• T. K. Prasad, M. V. Rajasekharan, [M(dipicH2)(H2O)3]2+, M = Ni, Cu, Zn (dipicH2 = dipicolinic acid) A combined crystallographic, spectroscopic and computational study. Polyhedron 2007, 26, 1364-1372.
• O. O. E. Onawumi, O. O. P. Faboya, O. A. Odunola, T. K. Prasad, M. V. Rajasekharan,Solvent water tapes in two hydrates of mu-oxo-bis[bis(2,2'-bipyridine)-(sulfato)iron(III)]. Acta. Cryst. 2006, C62, m480-m483.
• T. K. Prasad, M. V. Rajasekharan, A Novel Water Octamer in Ce(dipic)2(H2O)3.4H2O: Crystallographic, Thermal, and Theoretical Studies. Cryst. Growth. Des. 2006, 6, 488-491.
• M. A. H. Khan, T. K. Prasad, M. V. Rajasekharan, Silver(I) complexes of 2-(2-amino-ethyl)pyridine: nitrate and per-chlorate salts of bis[2-(2-amino-ethyl)pyridine] disilver(I). Acta. Cryst. 2005, C61, m281-m283.
• T. K. Prasad, M. V. Rajasekharan, Inter-conversion of 1-D coordination polymers in the Ce-Sr-dipicH2 system (dipicH2 = dipicolinic acid). Inorg. Chem. Commun. 2005, 8, 1116-1119.
• T. K. Prasad, S. Sailaja, M. V. Rajasekharan, 1-Dimensional and 2-dimensional coordination network structures in the Ba-Ce-dipicH2 system (dipicH2 = dipicolinic acid). Polyhedron 2005, 24, 1487-1496.

Book chapter

• T. K. Prasad, S. Bairy, N. Kamariah, Characterization of FOXO3-14-3-3 Interaction by Isothermal Titration Calorimetry. In FOXO Transcription Factors: Methods and Protocols; Link, W., Ed.; Springer US: New York, NY, 2025; pp 107–114.

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